By 55% to 45%, a majority of voters rejected the possibility of Scotland breaking away and becoming an independent nation, CNN reported.
Shortly afterward, Alex Salmond, the Scottish first minister and leader of the pro-independence Scottish National Party, announced his resignation from both posts, effective in November.
Salmond said he was proud of the campaign for independence, and that now was the time to hold the UK leadership to its promises of shifting more autonomy to Scotland.
"We now have the opportunity to hold Westminster's feet to the fire on the 'vow' that they have made to devolve further meaningful power to Scotland," he said in a statement. "This places Scotland in a very strong position."
UK Prime Minister David Cameron welcomed Scotland's decision in a televised statement outside 10 Downing Street, saying it was a clear result.
"Like millions of other people, I am delighted," he said.
Cameron said he would have been heartbroken to see the United Kingdom broken up -- but paid tribute to the efforts of both sides in the campaign.
Queen Elizabeth II said the outcome of the vote will be met with a range of emotions, but was hopeful "that these emotions will be tempered by an understanding of the feelings of others."
"Now, as we move forward, we should remember that despite the range of views that have been expressed, we have in common an enduring love of Scotland, which is one of the things that helps to unite us all," the Queen said in a statement.(KH)